stem cell ecm t75 Search Results


prostate cancer stem cells  (Celprogen Inc)
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Celprogen Inc prostate cancer stem cells
Prostate Cancer Stem Cells, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human hair follicle stem cells  (Celprogen Inc)
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Celprogen Inc human hair follicle stem cells
Human Hair Follicle Stem Cells, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human pancreatic cancer stem cells  (Celprogen Inc)
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Celprogen Inc human pancreatic cancer stem cells
Human Pancreatic Cancer Stem Cells, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stem cell ecm t75  (Celprogen Inc)
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Celprogen Inc stem cell ecm t75
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total rna  (Celprogen Inc)
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Celprogen Inc total rna
Total Rna, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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csc models  (Celprogen Inc)
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Celprogen Inc csc models
Csc Models, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lung cscs  (Celprogen Inc)
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Celprogen Inc lung cscs
Lung Cscs, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human renal stem cells  (Celprogen Inc)
90
Celprogen Inc human renal stem cells
Flow cytometric analysis of cell surface marker expression on mesenchymal <t>stem</t> <t>cells.</t> The values were normalized to the isotype IgG control. Expression of these markers was compared to the levels expressed at week 0 (in the undifferentiated phase before induction of <t>renal-lineage</t> differentiation). For urine-derived stem cells (USCs), 95% or more expressed the mesenchymal stem cell markers CD44 and CD73, whereas <3% expressed the hematopoietic lineage markers CD34 and CD45. Ctrl renal stem cells; ADSC adipose tissue-derived stem cells; AFSC amniotic fluid-derived stem cells; USC urine-derived stem cells
Human Renal Stem Cells, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human adult gscs  (Celprogen Inc)
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Celprogen Inc human adult gscs
Flow cytometric analysis of cell surface marker expression on mesenchymal <t>stem</t> <t>cells.</t> The values were normalized to the isotype IgG control. Expression of these markers was compared to the levels expressed at week 0 (in the undifferentiated phase before induction of <t>renal-lineage</t> differentiation). For urine-derived stem cells (USCs), 95% or more expressed the mesenchymal stem cell markers CD44 and CD73, whereas <3% expressed the hematopoietic lineage markers CD34 and CD45. Ctrl renal stem cells; ADSC adipose tissue-derived stem cells; AFSC amniotic fluid-derived stem cells; USC urine-derived stem cells
Human Adult Gscs, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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t25/t75 flasks corning  (Corning Life Sciences)
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Corning Life Sciences t25/t75 flasks corning
Flow cytometric analysis of cell surface marker expression on mesenchymal <t>stem</t> <t>cells.</t> The values were normalized to the isotype IgG control. Expression of these markers was compared to the levels expressed at week 0 (in the undifferentiated phase before induction of <t>renal-lineage</t> differentiation). For urine-derived stem cells (USCs), 95% or more expressed the mesenchymal stem cell markers CD44 and CD73, whereas <3% expressed the hematopoietic lineage markers CD34 and CD45. Ctrl renal stem cells; ADSC adipose tissue-derived stem cells; AFSC amniotic fluid-derived stem cells; USC urine-derived stem cells
T25/T75 Flasks Corning, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flasks celprogen cat no e36104 41 t75  (Celprogen Inc)
93
Celprogen Inc flasks celprogen cat no e36104 41 t75
Flow cytometric analysis of cell surface marker expression on mesenchymal <t>stem</t> <t>cells.</t> The values were normalized to the isotype IgG control. Expression of these markers was compared to the levels expressed at week 0 (in the undifferentiated phase before induction of <t>renal-lineage</t> differentiation). For urine-derived stem cells (USCs), 95% or more expressed the mesenchymal stem cell markers CD44 and CD73, whereas <3% expressed the hematopoietic lineage markers CD34 and CD45. Ctrl renal stem cells; ADSC adipose tissue-derived stem cells; AFSC amniotic fluid-derived stem cells; USC urine-derived stem cells
Flasks Celprogen Cat No E36104 41 T75, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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extracellular matrix pre coated t75 flasks  (Celprogen Inc)
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Celprogen Inc extracellular matrix pre coated t75 flasks
Flow cytometric analysis of cell surface marker expression on mesenchymal <t>stem</t> <t>cells.</t> The values were normalized to the isotype IgG control. Expression of these markers was compared to the levels expressed at week 0 (in the undifferentiated phase before induction of <t>renal-lineage</t> differentiation). For urine-derived stem cells (USCs), 95% or more expressed the mesenchymal stem cell markers CD44 and CD73, whereas <3% expressed the hematopoietic lineage markers CD34 and CD45. Ctrl renal stem cells; ADSC adipose tissue-derived stem cells; AFSC amniotic fluid-derived stem cells; USC urine-derived stem cells
Extracellular Matrix Pre Coated T75 Flasks, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Flow cytometric analysis of cell surface marker expression on mesenchymal stem cells. The values were normalized to the isotype IgG control. Expression of these markers was compared to the levels expressed at week 0 (in the undifferentiated phase before induction of renal-lineage differentiation). For urine-derived stem cells (USCs), 95% or more expressed the mesenchymal stem cell markers CD44 and CD73, whereas <3% expressed the hematopoietic lineage markers CD34 and CD45. Ctrl renal stem cells; ADSC adipose tissue-derived stem cells; AFSC amniotic fluid-derived stem cells; USC urine-derived stem cells

Journal: Tissue Engineering and Regenerative Medicine

Article Title: Potency of Human Urine-Derived Stem Cells for Renal Lineage Differentiation

doi: 10.1007/s13770-017-0081-y

Figure Lengend Snippet: Flow cytometric analysis of cell surface marker expression on mesenchymal stem cells. The values were normalized to the isotype IgG control. Expression of these markers was compared to the levels expressed at week 0 (in the undifferentiated phase before induction of renal-lineage differentiation). For urine-derived stem cells (USCs), 95% or more expressed the mesenchymal stem cell markers CD44 and CD73, whereas <3% expressed the hematopoietic lineage markers CD34 and CD45. Ctrl renal stem cells; ADSC adipose tissue-derived stem cells; AFSC amniotic fluid-derived stem cells; USC urine-derived stem cells

Article Snippet: Human renal stem cells (Cat. no. 36100-27; RSCs; Celprogen, Torrance, CA, USA) were used as an indicator cell line for renal lineage induction and were cultured in human kidney stem cell medium (Cat. no. M36100-27S; Celprogen).

Techniques: Marker, Expressing, Derivative Assay

Characterization of in vitro renal lineage-differentiated cells by morphological, immunocytochemical (ICC), quantitative real-time PCR, and secreted trophic factors analysis for 3 weeks. A Representative images from the morphological analysis. The original cell morphology (spindle-like) of the urine-derived stem cells (USCs) gradually changed to a large, round phenotype with a cobble stone-like appearance and cell aggregation. Representative ICC images using SSEA4, Pax2, Wt1, and Cadherin-6 antibodies. B In USCs, the stem cell marker SSEA4 was strongly expressed in undifferentiated stem cells, and then diminished in the differentiated phase. C Pax2 showed widespread nuclear expression at week 0, and then expression gradually became more localized and increased over time, and it was highly expressed in the cell aggregates at week 3. D Wt1 expression in the cytosol gradually increased over time. E Cadherin-6 was expressed in the cytoplasm of a few cells. The target proteins are shown in red, and the nucleus was stained with 4,6–diamidino-2–phenylindole (DAPI, blue). F Real-time PCR analysis. USCs showed the highest expression of LIM1, CD24, and OCLN. G Secreted trophic factor analysis by ELISA. VEGF and PDGF-bb were more strongly expressed in USCs than in ADSCs and AFSCs. The different letters on top of the bars indicate significant differences at p < 0.05. Ctrl renal stem cells, ADSC adipose tissue-derived stem cells, AFSC amniotic fluid-derived stem cells, USC urine-derived stem cells. (Color figure online)

Journal: Tissue Engineering and Regenerative Medicine

Article Title: Potency of Human Urine-Derived Stem Cells for Renal Lineage Differentiation

doi: 10.1007/s13770-017-0081-y

Figure Lengend Snippet: Characterization of in vitro renal lineage-differentiated cells by morphological, immunocytochemical (ICC), quantitative real-time PCR, and secreted trophic factors analysis for 3 weeks. A Representative images from the morphological analysis. The original cell morphology (spindle-like) of the urine-derived stem cells (USCs) gradually changed to a large, round phenotype with a cobble stone-like appearance and cell aggregation. Representative ICC images using SSEA4, Pax2, Wt1, and Cadherin-6 antibodies. B In USCs, the stem cell marker SSEA4 was strongly expressed in undifferentiated stem cells, and then diminished in the differentiated phase. C Pax2 showed widespread nuclear expression at week 0, and then expression gradually became more localized and increased over time, and it was highly expressed in the cell aggregates at week 3. D Wt1 expression in the cytosol gradually increased over time. E Cadherin-6 was expressed in the cytoplasm of a few cells. The target proteins are shown in red, and the nucleus was stained with 4,6–diamidino-2–phenylindole (DAPI, blue). F Real-time PCR analysis. USCs showed the highest expression of LIM1, CD24, and OCLN. G Secreted trophic factor analysis by ELISA. VEGF and PDGF-bb were more strongly expressed in USCs than in ADSCs and AFSCs. The different letters on top of the bars indicate significant differences at p < 0.05. Ctrl renal stem cells, ADSC adipose tissue-derived stem cells, AFSC amniotic fluid-derived stem cells, USC urine-derived stem cells. (Color figure online)

Article Snippet: Human renal stem cells (Cat. no. 36100-27; RSCs; Celprogen, Torrance, CA, USA) were used as an indicator cell line for renal lineage induction and were cultured in human kidney stem cell medium (Cat. no. M36100-27S; Celprogen).

Techniques: In Vitro, Real-time Polymerase Chain Reaction, Derivative Assay, Marker, Expressing, Staining, Enzyme-linked Immunosorbent Assay

In vivo safety analysis of renal-differentiated cells. Renal lineage-differentiated USCs were implanted into the subcapsule of the kidney. Four weeks later, a histological analysis was performed. No abnormal morphology was observed in the implanted kidneys. Ctrl renal stem cells, ADSC adipose tissue-derived stem cells, AFSC amniotic fluid-derived stem cells, USC urine-derived stem cells

Journal: Tissue Engineering and Regenerative Medicine

Article Title: Potency of Human Urine-Derived Stem Cells for Renal Lineage Differentiation

doi: 10.1007/s13770-017-0081-y

Figure Lengend Snippet: In vivo safety analysis of renal-differentiated cells. Renal lineage-differentiated USCs were implanted into the subcapsule of the kidney. Four weeks later, a histological analysis was performed. No abnormal morphology was observed in the implanted kidneys. Ctrl renal stem cells, ADSC adipose tissue-derived stem cells, AFSC amniotic fluid-derived stem cells, USC urine-derived stem cells

Article Snippet: Human renal stem cells (Cat. no. 36100-27; RSCs; Celprogen, Torrance, CA, USA) were used as an indicator cell line for renal lineage induction and were cultured in human kidney stem cell medium (Cat. no. M36100-27S; Celprogen).

Techniques: In Vivo, Derivative Assay